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Chitin and its derivatives (chitosan and glucosamine) were studied for their effects on ethanol production using YPD (yeast extract 10%, peptone 20%, glucose 20%, agar 20%) medium. All chitin derivatives, particularly chitin, increased ethanol production compared with control. In non-steamed alcohol fermentation of tapioca, addition of 0.9% chitin yielded higher ethanol production (13.6%) with lower acetaldehyde (21.91ppm) and methanol (65.49ppm) contents than those (12.7%, 35.05ppm, 84.21ppm, respectively) of control after fermentation for 120 hr at 30℃. Results indicate that chitin can be used to increase ethanol production in non-steamed alcohol fermentation of tapioca.
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